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DNA ligase is an essential enzyme that joins breaks in the DNA backbone by forming phosphodiester bonds between adjacent nucleotides. It is crucial for maintaining the continuity and stability of DNA molecules. 🔬 Function DNA ligase seals nicks (gaps) that occur between the 3'-hydroxyl (–OH) end of one nucleotide and the 5'-phosphate (–PO₄) end of another. This process is vital in: DNA replication – joins Okazaki fragments on the lagging strand. DNA repair – fixes single-strand breaks caused by
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